Part:BBa_K133002:Design

CF-RGD_Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1207
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 764
  Illegal BamHI site found at 1275
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1558
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

The composite parts of chimeric protein were amplificated by PCR reaction from E. coli and H. pylori genomic DNA respecively.